BAD antibody (pSer112)

Catalog No. ABIN1842343

Product Details anti-BAD Antibody

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Binding Specificity: pSer112

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Rabbit Anti-BAD Polyclonal Antibody detects endogenous levels of total BAD protein.

Characteristics: Molecular Weight: Predicted Band Size 23 kDa, Observed Band Size 23 kDa

Purification: The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen.

Immunogen: Synthesized non-phosphopeptide derived from human BAD around pSer112 (H-S-SP-Y-P)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:1,000,
IHC: 1:50-1:100

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20 °C for 1 year.

References for anti-BAD antibody (ABIN1842343)

Marchion, Cottrill, Xiong, Chen, Bicaku, Fulp, Bansal, Chon, Stickles, Kamath, Hakam, Li, Su, Moreno, Judson, Berchuck, Wenham, Apte, Gonzalez-Bosquet, Bloom, Eschrich, Sebti, Chen, Lancaster: "BAD phosphorylation determines ovarian cancer chemosensitivity and patient survival." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 19, pp. 6356-66, (2011) (PubMed).

Target Details for BAD

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Alternative Name: BAD (BAD Products)

Synonyms: bad antibody, fa01b12 antibody, wu:fa01b12 antibody, wu:fa96d04 antibody, BAD antibody, BBC2 antibody, BCL2L8 antibody, AI325008 antibody, Bbc2 antibody, BCL2-associated agonist of cell death b antibody, BCL2 associated agonist of cell death antibody, BCL2-associated agonist of cell death antibody, badb antibody, BAD antibody, Bad antibody

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events