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SMAD3 antibody (pSer425)

SMAD3 Reactivity: Human, Mouse, Rat IHC, IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1842543
  • Target See all SMAD3 Antibodies
    SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
    Binding Specificity
    • 34
    • 29
    • 28
    • 28
    • 20
    • 14
    • 14
    • 11
    • 11
    • 9
    • 8
    • 7
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer425
    Reactivity
    • 257
    • 150
    • 138
    • 28
    • 10
    • 9
    • 8
    • 8
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 248
    • 23
    Rabbit
    Clonality
    • 231
    • 40
    Polyclonal
    Conjugate
    • 138
    • 19
    • 14
    • 14
    • 12
    • 8
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    This SMAD3 antibody is un-conjugated
    Application
    • 231
    • 117
    • 108
    • 74
    • 59
    • 55
    • 55
    • 53
    • 28
    • 13
    • 10
    • 8
    • 5
    • 4
    • 2
    • 1
    Immunohistochemistry (IHC), Immunofluorescence (IF)
    Specificity
    Rabbit Anti-Smad3 Polyclonal Antibody detects endogenous levels of total Smad3 protein.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
    Immunogen
    Synthesized non-phosphopeptide derived from human Smad3 around pSer425 (C-S-S-V-SP).
    Isotype
    IgG
    Top Product
    Discover our top product SMAD3 Primary Antibody
  • Application Notes
    IHC: 1:50-1:100,
    IF: 1:100-1:200
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.,
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C for 1 year
  • Tang, Yamashita, Coussens, Tang, Wang, Li, Deng, Cheng, Zhang: "Ablation of Smurf2 reveals an inhibition in TGF-? signalling through multiple mono-ubiquitination of Smad3." in: The EMBO journal, Vol. 30, Issue 23, pp. 4777-89, (2011) (PubMed).

  • Target
    SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
    Alternative Name
    Smad3 (SMAD3 Products)
    Synonyms
    HSPC193 antibody, HsT17436 antibody, JV15-2 antibody, LDS1C antibody, LDS3 antibody, MADH3 antibody, madh3 antibody, madh3a antibody, smad3 antibody, wu:fa99e03 antibody, XSmad3 antibody, XenMLP antibody, Xmad3 antibody, madh3-A antibody, Madh3 antibody, AU022421 antibody, Smad 3 antibody, mad3 antibody, madh3b antibody, zgc:92234 antibody, SMAD family member 3 antibody, SMAD family member 3a antibody, SMAD family member 3 L homeolog antibody, SMAD family member 3b antibody, SMAD3 antibody, smad3a antibody, smad3.L antibody, Smad3 antibody, smad3b antibody
    Pathways
    Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
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