PTPN11 antibody (C-Term)

Catalog No. ABIN184604

The Goat Polyclonal anti-PTPN11 antibody has been validated for WB, ELISA, IF and FACS. It is suitable to detect PTPN11 in samples from Human.

Quick Overview for PTPN11 antibody (C-Term) (ABIN184604)

Target: PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PTPN11 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-PTPN11 Antibody

Binding Specificity: C-Term

Purpose: SHP2 / PTPN11

Sequence: YENVGLMQQQ KSFR

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-YENVGLMQQQKSFR, from the C Terminus of the protein sequence according to NP_002825.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in Human Muscle lysates (calculated MW of 68.0 kDa according to NP_002825.3). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PTPN11

Target: PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))

Alternative Name: PTPN11

Background: PTPN11, SHP2, protein tyrosine phosphatase, non-receptor type 11 (Noonan syndrome 1), CFC, NS1, BPTP3, PTP2C, SHP-2, PTP-1D, SH-PTP2, SH-PTP3, Noonan syndrome 1, protein-tyrosine phosphatase 2C, protein tyrosine phosphatase, non-receptor type 11, MGC14433

Gene ID: 5781, 19247, 25622

NCBI Accession: NP_002825, NP_001317366

Pathways: JAK-STAT Signaling, RTK Signaling, TCR Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, Warburg Effect