ELMO2 antibody (C-Term)

Catalog No. ABIN184684

Product Details anti-ELMO2 Antibody

Target: ELMO2 (Engulfment and Cell Motility 2 (ELMO2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ELMO2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: ELMO2

Sequence: PKEPSSYDFV YHYG

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: C-PKEPSSYDFVYHYG

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Prostate shows pixulated staining in the cytoplasm of stroma cells. Recommended concentration: 3-6 μg/mL.

Western Blot: Approx 80 kDa band observed in lysates of cell lines HEK293, Jurkat, RAW264.7 and in Human Cerebellum and Rat Brain lysates (calculated MW of 82.6 kDa according to Human NP_573403.1 and NP_877496.1 and Mouse NP_525026.2, and 83.7 kDa according

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in A549 and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ELMO2

Target: ELMO2 (Engulfment and Cell Motility 2 (ELMO2))

Alternative Name: ELMO2 (ELMO2 Products)

Synonyms: CED-12 antibody, CED12 antibody, ELMO-2 antibody, ELMO2 antibody, MGC114811 antibody, 1190002F24Rik antibody, mKIAA1834 antibody, engulfment and cell motility 2 antibody, engulfment and cell motility protein 2 antibody, engulfment and cell motility 2 L homeolog antibody, ELMO2 antibody, LOC100232489 antibody, Elmo2 antibody, elmo2.L antibody, elmo2 antibody

Background: ELMO2, engulfment and cell motility 2 (ced-12 homolog, C. elegans), CED12, FLJ11656, KIAA1834, ced-12 homolog 2, PH domain protein CED12A, engulfment and cell motility 2, engulfment and cell motility 2, RP11-394O2.2, CED-12, ELMO-2

Gene ID: 63916, 140579

NCBI Accession: NP_877496, NP_573403