DAP3 antibody (C-Term)

Catalog No. ABIN184743

Product Details anti-DAP3 Antibody

Target: DAP3 (Death-Associated Protein 3 (DAP3))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DAP3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Purpose: DAP3

Sequence: NPSLLERHCA YL

Specificity: This antibody is expected to recognise isoform 1 (NP_387506.1), isoform 2 (NP_001186779.1) and isoform 3 (NP_001186780.1). Reported variants represent identical protein (NP_387506.1, NP_004623.1, NP_001186778.1).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence NPSLLERHCAYL, from the C Terminus of the protein sequence according to NP_387506.1, NP_001186779.1, NP_001186780.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 40 kDa band observed in lysates of cell lines HeLa and HepG2 and in Human Kidney lysates (calculated MW of 41 kDa according to NP_001186779.1 and NP_001186780). Recommended concentration: 0.1-1 μg/mL. Primary incubated 1 hour at room

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DAP3

Target: DAP3 (Death-Associated Protein 3 (DAP3))

Alternative Name: DAP3 (DAP3 Products)

Background: DAP3, death associated protein 3, DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29, bMRP-10, death-associated protein 3, mitochondrial 28S ribosomal protein S29

Gene ID: 7818

NCBI Accession: NP_387506, NP_001186779, NP_001186780