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ARPC2 antibody (C-Term)

Verified ARPC2 Reactivity: Human WB, ELISA, IHC, IF Host: Goat Polyclonal unconjugated
Catalog No. ABIN184748
  • Target See all ARPC2 Antibodies
    ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))
    Binding Specificity
    • 15
    • 14
    • 7
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 57
    • 13
    • 11
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 53
    • 4
    Goat
    Clonality
    • 51
    • 6
    Polyclonal
    Conjugate
    • 22
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This ARPC2 antibody is un-conjugated
    Application
    • 45
    • 22
    • 13
    • 13
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
    Purpose
    ARPC2
    Sequence
    EMKTITGKTF SSR
    Specificity
    The reported variants NP_005722.1 and NP_690601.1 represent identical protein.
    Cross-Reactivity
    Cow, Dog, Human, Mouse, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-EMKTITGKTFSSR, from the C Terminus of the protein sequence according to NP_005722.1, NP_690601.1.
    Isotype
    IgG
    Top Product
    Discover our top product ARPC2 Primary Antibody
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration, 3-5 μg/mL.

    Western Blot: Approx 34-35 kDa band observed in lysates of cell lines CACO-2, HeLa and Jurkat (calculated MW of 34.3 kDa according to NP_005722.1). Recommended concentration: 0.1-0.5 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Sakata, Taniguchi, Yasuda, Adachi-Morishima, Hamazaki, Nakayama, Miki, Minato, Narumiya: "Impaired T lymphocyte trafficking in mice deficient in an actin-nucleating protein, mDia1." in: The Journal of experimental medicine, Vol. 204, Issue 9, pp. 2031-8, (2007) (PubMed).

  • Target
    ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))
    Alternative Name
    ARPC2 (ARPC2 Products)
    Synonyms
    arc34 antibody, p34-arc antibody, pnas-139 antibody, pro2446 antibody, Arpc2 antibody, Arc-p34 antibody, 2210023N03Rik antibody, 34kDa antibody, p34-Arc antibody, fk84a07 antibody, wu:fa22f07 antibody, wu:fk84a07 antibody, zgc:77769 antibody, ARC34 antibody, PNAS-139 antibody, actin related protein 2/3 complex subunit 2 antibody, actin related protein 2/3 complex, subunit 2, 34kDa antibody, actin related protein 2/3 complex, subunit 2 antibody, actin related protein 2/3 complex subunit 2 S homeolog antibody, ARPC2 antibody, arpc2 antibody, Arpc2 antibody, arpc2.S antibody
    Background
    ARPC2, actin related protein 2/3 complex, subunit 2, 34 kDa, ARC34, PRO2446, p34-Arc, PNAS-139, ARP2/3 protein compex subunit 34, actin related protein 2/3 complex subunit 2
    Gene ID
    10109, 76709
    NCBI Accession
    NP_005722, NP_690601
    Pathways
    RTK Signaling, Regulation of Actin Filament Polymerization
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