JUP antibody (C-Term)

Catalog No. ABIN184775

Product Details anti-JUP Antibody

Target: JUP (Junction Plakoglobin (JUP))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This JUP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: Plakoglobin / Gamma-catenin

Sequence: GLRPPYPTAD HMLA

Specificity: This antibody is expected to recognise both reported isoforms, as represented by NP_002221.1 and NP_068831.1.

Cross-Reactivity: Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-GLRPPYPTADHMLA, from the C Terminus of the protein sequence according to NP_002221.1, NP_068831.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Heart. Recommended concentration: 5 μg/mL.

Western Blot: Approx 80 kDa band observed in lysates of cell lines A431 and NIH3T3 and in Rat Eye lysates (calculated MW of 81.7 kDa according to Human NP_002221.1 and Mouse NP_034723.1). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for JUP

Target: JUP (Junction Plakoglobin (JUP))

Alternative Name: JUP (JUP Products)

Synonyms: ARVD12 antibody, CTNNG antibody, DP3 antibody, DPIII antibody, PDGB antibody, PKGB antibody, arvd12 antibody, ctnng antibody, dp3 antibody, dpiii antibody, pdgb antibody, pkgb antibody, plak antibody, D930025P04Rik antibody, PG antibody, cb80 antibody, danPG antibody, fb11b06 antibody, jup antibody, wu:fb11b06 antibody, junction plakoglobin antibody, junction plakoglobin L homeolog antibody, Junction plakoglobin antibody, junction plakoglobin a antibody, junction plakoglobin S homeolog antibody, JUP antibody, Jup antibody, jup antibody, jup.L antibody, Tsp_06656 antibody, plak antibody, jupa antibody, jup.S antibody

Background: JUP, GAMMA-CATENIN, gamma-catenin, junction plakoglobin, DP3, PDGB, PKGB, DPIII, ARVD12, CTNNG, OTTHUMP00000164735, catenin (cadherin-associated protein), gamma (80kD), catenin (cadherin-associated protein), gamma 80 kDa

Gene ID: 3728, 16480

NCBI Accession: NP_002221, NP_068831

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location