NDC80 antibody (C-Term)

Catalog No. ABIN184821

Product Details anti-NDC80 Antibody

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NDC80 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: HEC1

Sequence: YEKKATLIKS SEE

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: C-YEKKATLIKSSEE

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Liver and Adrenal Gland. Recommended concentration: 5 μg/mL.

Western Blot: Approx 75-80 kDa band observed in Hela lysate (predicted MW of 74 kDa according to NP_006092). Recommended for use at 0.5-2 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Alternative Name: NDC80 (NDC80 Products)

Synonyms: CG9938 antibody, Dmel\\CG9938 antibody, Hec1 antibody, NDC80 antibody, dNdc80 antibody, dmNdc80 antibody, hec1 antibody, KNTC2 antibody, ndc10 antibody, tid3 antibody, 2610020P18Rik antibody, AV139730 antibody, HEC antibody, HEC1 antibody, Kntc2 antibody, kntc2l antibody, wu:fb52e02 antibody, zgc:111801 antibody, HsHec1 antibody, TID3 antibody, hsNDC80 antibody, kntc2 antibody, NDC80, kinetochore complex component antibody, CG9938 gene product from transcript CG9938-RA antibody, spindle pole body protein Ndc80 antibody, kinetochore protein Ndc80 antibody, NDC80 homolog, kinetochore complex component (S. cerevisiae) antibody, NDC80 kinetochore complex component antibody, NDC80 kinetochore complex component S homeolog antibody, NDC80 antibody, Ndc80 antibody, ndc80 antibody, ndc80.S antibody

Background: NDC80, HEC1, HEC, highly expressed in cancer, rich in leucine heptad repeats, HEC-1, KNTC2, NDC80 homolog, kinetochore complex component (S. cerevisiae), TID3, hsNDC80, kinetochore associated 2

Gene ID: 10403

NCBI Accession: NP_006092

Pathways: Maintenance of Protein Location