CLPP antibody (C-Term)

Catalog No. ABIN184946

The Goat Polyclonal anti-CLPP antibody has been validated for WB, ELISA and IHC. It is suitable to detect CLPP in samples from Human.

Quick Overview for CLPP antibody (C-Term) (ABIN184946)

Target: CLPP (ClpP Caseinolytic Peptidase, ATP-Dependent, Proteolytic Subunit Homolog (E. Coli) (CLPP))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CLPP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CLPP Antibody

Binding Specificity: C-Term

Purpose: CLPP

Sequence: EAAPAAEPVP AST

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EAAPAAEPVPAST, from the C Terminus of the protein sequence according to NP_006003.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Liver shows cytoplasmic staining consistent with mitochondrai in hepatocytes. Recommended concentration, 2-4 μg/mL.

Western Blot: Approx 26 kDa band observed in Human Heart (calculated MW of 30.2 kDa according to NP_006003.1). Recommended for use at 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CLPP

Target: CLPP (ClpP Caseinolytic Peptidase, ATP-Dependent, Proteolytic Subunit Homolog (E. Coli) (CLPP))

Alternative Name: CLPP

Background: CLPP, ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog (E. coli), ATP-dependent proteolytic subunit E.coli homolog, ATP-dependent protease ClpAP (E. coli), proteolytic subunit, human, ClpP (caseinolytic protease, ATP-dependent, prote

Gene ID: 8192

NCBI Accession: NP_006003