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PPP1R8 antibody (C-Term)

Verified PPP1R8 Reactivity: Human, Mouse WB, ELISA, IHC Host: Goat Polyclonal unconjugated
Catalog No. ABIN185050
  • Target See all PPP1R8 Antibodies
    PPP1R8 (Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8))
    Binding Specificity
    • 15
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 40
    • 32
    • 17
    • 17
    • 8
    • 8
    • 7
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    Human, Mouse
    Host
    • 40
    • 6
    • 2
    • 1
    Goat
    Clonality
    • 43
    • 6
    Polyclonal
    Conjugate
    • 28
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PPP1R8 antibody is un-conjugated
    Application
    • 41
    • 14
    • 13
    • 13
    • 9
    • 6
    • 5
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Purpose
    NIPP1 / PPP1R8
    Sequence
    EAWPGKKPTP SLLI
    Specificity
    This antibody is expected to recognise all three human isoforms of this protein.
    Cross-Reactivity
    Cow, Dog, Human, Mouse
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-EAWPGKKPTPSLLI, from the C Terminus of the protein sequence according to NP_002704.1, NP_054829.2, NP_612568.1.
    Isotype
    IgG
    Top Product
    Discover our top product PPP1R8 Primary Antibody
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Thymus shows speckled staining of nuclei. Recommended concentration: 2.5-3.8 μg/mL.

    Western Blot: Approx 40 kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat and NIH3T3 (calculated MW of 38.5 kDa according to Human NP_054829.2 and to Mouse NP_666266.1). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 h

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    PPP1R8 (Protein Phosphatase 1, Regulatory Subunit 8 (PPP1R8))
    Alternative Name
    PPP1R8 (PPP1R8 Products)
    Synonyms
    ARD-1 antibody, ARD1 antibody, NIPP-1 antibody, NIPP1 antibody, PRO2047 antibody, 6330548N22Rik antibody, AU044684 antibody, PPP1R8 antibody, ard-1 antibody, ard1 antibody, nipp-1 antibody, nipp1 antibody, ppp1r8 antibody, si:ch211-206a7.1 antibody, protein phosphatase 1 regulatory subunit 8 antibody, protein phosphatase 1, regulatory subunit 8 antibody, protein phosphatase 1, regulatory (inhibitor) subunit 8 antibody, protein phosphatase 1, regulatory subunit 8b antibody, protein phosphatase 1 regulatory subunit 8 L homeolog antibody, protein phosphatase 1, regulatory subunit 8a antibody, protein phosphatase 1 regulatory subunit 8 S homeolog antibody, PPP1R8 antibody, Ppp1r8 antibody, ppp1r8b antibody, ppp1r8.L antibody, ppp1r8a antibody, ppp1r8.S antibody
    Background
    PPP1R8, NIPP1, protein phosphatase 1, regulatory (inhibitor) subunit 8, ARD1, NIPP-1, PRO2047, ard-1, activator of RNA decay, nuclear subunit of PP-1, homolog of E.coli RNase E, nuclear inhibitor of protein phosphatase-1, protein phosphatase-1, regulatory
    Gene ID
    5511, 100336
    NCBI Accession
    NP_002704, NP_054829, NP_612568
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