Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3) (AA 429-442), (AA 443-456) antibody

Catalog No. ABIN185294

Product Details

Target: Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3)

Binding Specificity: AA 429-442, AA 443-456

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Application: ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Purpose: LXR alpha / LXR beta

Sequence: CRLQDKKLPP LLSEI

Specificity: This antibody is expected to recognise epitopes including aa 429-442 of human LXR alpha protein (NP_005684.2) and aa 443-456 of human LXR beta protein (NP_009052.3).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence CRLQDKKLPPLLSEI, from the internal region of the protein sequence according to NP_005684.2, NP_009052.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in Human, Liver and Kidney lysates and in preliminary testing of Human Spleen lysate (calculated MW of 51.1 kDa according to NP_001238863.1). This band was successfully blocked by incubation with the immunizing pe

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of A549 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details

Target: Nuclear Receptor Subfamily 1, Group H, Member 2/3 (NR1H2/3)

Alternative Name: NR1H3, NR1H2

Synonyms: LXR-a antibody, LXRA antibody, RLD-1 antibody, nuclear receptor subfamily 1 group H member 3 antibody, NR1H3 antibody

Background: NR1H3, LXRA, LXR-a, RLD-1, nuclear receptor subfamily 1, group H, member 3, liver X receptor, alpha, NR1H2, NER, UNR, LXR-b, NER-I, RIP15, nuclear receptor subfamily 1, group H, member 2, ubiquitously-expressed nuclear receptor / NR1H2, NER, UNR, LXR-b, N

Gene ID: 10062, 7376

NCBI Accession: NP_005684, NP_009052, NP_001123573, NP_001238863, NP_001350524

Pathways: Nuclear Receptor Transcription Pathway, Cellular Response to Molecule of Bacterial Origin, Hepatitis C