AHI1 antibody (N-Term)

Catalog No. ABIN185303

Product Details anti-AHI1 Antibody

Target: AHI1 (Abelson Helper Integration Site 1 (AHI1))

Binding Specificity: N-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AHI1 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Purpose: AHI1 / Jouberin

Sequence: PTAESEAKVK TC

Predicted Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence PTAESEAKVKTC, from the N Terminus of the protein sequence according to NP_060121.3, NP_001128303.1, NP_001128302.1, NP_001128304.1.

Isotype: IgG

Application Details

Application Notes:

DS WB Results: Preliminary experiments gave a consistent band at approx 75-80 kDa in Human Cerebellum and Frontal Cortex lysates after 0.5 μg/mL antibody staining and was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for this band, given the calculated size of 137 kDa according to NP_060121.3

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-AHI1 antibody (ABIN185303)

Kennah, Ringrose, Zhou, Esmailzadeh, Qian, Su, Zhou, Jiang: "Identification of tyrosine kinase, HCK, and tumor suppressor, BIN1, as potential mediators of AHI-1 oncogene in primary and transformed CTCL cells." in: Blood, Vol. 113, Issue 19, pp. 4646-55, (2009) (PubMed).

Zhou, Zhao, Ringrose, DeGeer, Kennah, Lin, Sheng, Li, Turhan, Jiang: "AHI-1 interacts with BCR-ABL and modulates BCR-ABL transforming activity and imatinib response of CML stem/progenitor cells." in: The Journal of experimental medicine, Vol. 205, Issue 11, pp. 2657-71, (2008) (PubMed).

Target Details for AHI1

Target: AHI1 (Abelson Helper Integration Site 1 (AHI1))

Alternative Name: AHI1 (AHI1 Products)

Synonyms: AHI1 antibody, AHI-1 antibody, JBTS3 antibody, ORF1 antibody, dJ71N10.1 antibody, 1700015F03Rik antibody, Ahi-1 antibody, D10Bwg0629e antibody, Abelson helper integration site 1 antibody, AHI1 antibody, ahi1 antibody, Ahi1 antibody

Background: AHI1, AHI-1, DKFZp686J1653, FLJ14023, FLJ20069, JBTS3, ORF1, dJ71N10.1, Abelson helper integration site, jouberin, Ahi-1 isoform I, OTTHUMP00000017263, OTTHUMP00000017264, OTTHUMP00000017265, contatins SH3 and WD40 domains, Abelson helper integration site 1

Gene ID: 54806

NCBI Accession: NP_060121, NP_001128304, NP_001337433