SEMA3E antibody (C-Term)

Catalog No. ABIN185359

Product Details anti-SEMA3E Antibody

Target: SEMA3E (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SEMA3E antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Purpose: SEMA3E

Sequence: KPEHYRLPRH TLDS

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-KPEHYRLPRHTLDS, from the C Terminus of the protein sequence according to NP_036563.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Adrenal Medulla shows granular cytoplasmic staining increasing towards the zona reticularis. Recommended concentration, 2-4 μg/mL.

Western Blot: Preliminary experiments gave bands at approx 35 kDa and 20 kDa in Human Prostate lysates after 0.1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculate

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SEMA3E

Target: SEMA3E (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E))

Alternative Name: SEMA3E (SEMA3E Products)

Synonyms: M-SEMAH antibody, M-SemaK antibody, SEMAH antibody, coll-5 antibody, 6430702L12 antibody, AA408817 antibody, Semah antibody, mKIAA0331 antibody, semaphorin 3E antibody, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E antibody, SEMA3E antibody, Sema3e antibody

Background: SEMA3E, SEMAH, coll-5, M-SEMAH, M-SemaK, KIAA0331, M-sema H, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3E

Gene ID: 9723

NCBI Accession: NP_036563