MIF antibody (C-Term)

Catalog No. ABIN185406

Product Details anti-MIF Antibody

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MIF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: MIF

Sequence: NAANVGWNNS TFA

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-NAANVGWNNSTFA, from the C Terminus of the protein sequence according to NP_002406.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Thymus. Recommended concentration: 5 μg/mL.

Western Blot: Approx 13 kDa band observed in Human Lymph Node, and Thymus lysates and similar bands are observed in lysates of cell lines Daudi and Jurkat (calculated MW of 12.5 kDa according to NP_002406.1). Recommended concentration: 0.01-0.03 μg/mL. Pri

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for MIF

Target: MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))

Alternative Name: MIF (MIF Products)

Synonyms: mif antibody, Mif antibody, gif antibody, glif antibody, mmif antibody, LOC100136498 antibody, LOC100284350 antibody, LOC100284546 antibody, GIF antibody, Glif antibody, GLIF antibody, MMIF antibody, macrophage migration inhibitory factor L homeolog antibody, macrophage migration inhibitory factor antibody, macrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody, mif.L antibody, mif antibody, MIF antibody, Mif antibody, PHATRDRAFT_49660 antibody, LOC100136498 antibody, cl405_1 antibody, LOC100284546 antibody

Background: MIF, macrophage migration inhibitory factor (glycosylation-inhibiting factor) , HGNC:7097, GIF, GLIF, MMIF, glycosylation-inhibiting factor, phenylpyruvate tautomerase, macrophage migration inhibitory factor

Gene ID: 4282

NCBI Accession: NP_002406

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling