ENPEP antibody (Internal Region)

Catalog No. ABIN185416

Product Details anti-ENPEP Antibody

Target: ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))

Binding Specificity: Internal Region

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ENPEP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Purpose: Aminopeptidase A

Sequence: EQYQKTSLAQ EKEK

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-EQYQKTSLAQEKEK, from the internal region of the protein sequence according to NP_001968.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows staining of basement membrane and the mesangium in the glomerulus. Recommended concentration: 3-5 μg/mL.

Western Blot: Approx 170 kDa band observed in Human Brain and Testis lysates (calculated MW of 109 kDa according to NP_001968.2). Primary incubation was for 1 hour.The observed molecular weight corresponds to earlier findings in literature with different

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ENPEP

Target: ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))

Alternative Name: ENPEP (ENPEP Products)

Synonyms: im:7152184 antibody, si:ch211-146m5.2 antibody, 6030431M22Rik antibody, APA antibody, Bp-1/6C3 antibody, Ly-51 antibody, Ly51 antibody, CD249 antibody, gp160 antibody, glutamyl aminopeptidase antibody, ENPEP antibody, enpep antibody, ECU01_1470 antibody, pepA antibody, LOC5568916 antibody, CpipJ_CPIJ011103 antibody, Eint_011390 antibody, Halhy_0222 antibody, Enpep antibody

Background: Aminopeptidase A, ENPEP, glutamyl aminopeptidase (aminopeptidase A) , HGNC:3355, APA, gp160, CD249, glutamyl aminopeptidase

Gene ID: 2028, 13809

NCBI Accession: NP_001968

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones