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GLI2 antibody (Internal Region)

Verified GLI2 Reactivity: Human ELISA, IHC Host: Goat Polyclonal unconjugated
Catalog No. ABIN185585
  • Target See all GLI2 Antibodies
    GLI2 (GLI Family Zinc Finger 2 (GLI2))
    Binding Specificity
    • 7
    • 7
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 40
    • 12
    • 4
    • 1
    • 1
    Human
    Host
    • 40
    • 2
    Goat
    Clonality
    • 40
    • 2
    Polyclonal
    Conjugate
    • 18
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    This GLI2 antibody is un-conjugated
    Application
    • 16
    • 9
    • 8
    • 5
    • 4
    • 3
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC)
    Purpose
    GLI2
    Sequence
    DLKEDLDRDD CKQE
    Specificity
    This antibody is expected to recognise all four reported isoforms (NP_084655.1, NP_084656.1, NP_084657.1 and NP_005261.1)
    Predicted Reactivity
    Human, Mouse
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence DLKEDLDRDDCKQE, from the internal region of the protein sequence according to NP_005261.2.
    Isotype
    IgG
    Top Product
    Discover our top product GLI2 Primary Antibody
  • Application Notes

    DS WB Results: Preliminary experiments gave an approx 55 kDa band in Human Bone Marrow and Ovary lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 168 kDa according to NP_005261.2. The 55 kDa band was successfully blocked by incubation with the immunizing peptide.

    DS IHC Results: In paraffin embedded Human Lymph Node shows nuclear staining in macrophages. Recommended concentration: 4-6 μg/mL.

    Peptide ELISA: antibody detection limit dilution 1:2000.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    GLI2 (GLI Family Zinc Finger 2 (GLI2))
    Alternative Name
    GLI2 (GLI2 Products)
    Synonyms
    HPE9 antibody, THP1 antibody, THP2 antibody, GLI2/GLI4 antibody, GLI3 antibody, AW546128 antibody, GLI family zinc finger 2 antibody, GLI-Kruppel family member GLI2 antibody, GLI2 antibody, Gli2 antibody
    Background
    GLI2, GLI-Kruppel family member GLI2 , THP2, oncogene GLI2, tax helper protein 2, tax-responsive element-2 holding protein, tax-responsive element-25-bp sequence binding protein, zinc finger protein GLI2
    Gene ID
    2736
    NCBI Accession
    NP_005261
    Pathways
    Hedgehog Signaling, Dopaminergic Neurogenesis
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