XRCC5 antibody (Internal Region)
-
- Target See all XRCC5 Antibodies
- XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))
-
Binding Specificity
- Internal Region
-
Reactivity
- Human
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- This XRCC5 antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Purpose
- Ku80 / XRCC5
- Sequence
- SLAKKDEKTD TLED
- Cross-Reactivity
- Human
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Immunogen
- Peptide with sequence C-SLAKKDEKTDTLED, from the internal region of the protein sequence according to NP_066964.1.
- Isotype
- IgG
- Top Product
- Discover our top product XRCC5 Primary Antibody
-
-
- Application Notes
-
Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 5 μg/mL.
Western Blot: Approx 80-85 kDa band observed in lysates of HeLa (calculated MW of 82.7 kDa according to NP_066964.1). Recommended concentration: 0.1-0.3 μg/mL. An additional band of 75 kDa was observed consistent with products from other sources.
Peptide ELISA: antibody detection limit dilution 1:16000.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
-
- Target
- XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining) (XRCC5))
- Alternative Name
- XRCC5 (XRCC5 Products)
- Synonyms
- AI314015 antibody, Ku80 antibody, Ku86 antibody, Kup80 antibody, KARP-1 antibody, KARP1 antibody, KU80 antibody, KUB2 antibody, NFIV antibody, xrcc5-a antibody, XRCC5 antibody, X-ray repair cross complementing 5 antibody, X-ray repair complementing defective repair in Chinese hamster cells 5 antibody, X-ray repair complementing defective repair in Chinese hamster cells 5 L homeolog antibody, XRCC5 antibody, Xrcc5 antibody, xrcc5.L antibody
- Background
- XRCC5, KU80, X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining Ku autoantigen, 80 kDa) , HGNC:12833, KARP-1, KARP1, Ku86, NFIV , ATP-dependent DNA helicase II, DNA repair protein XRCC5, Ku86 autoantigen r
- Gene ID
- 7520
- NCBI Accession
- NP_066964
- Pathways
- DNA Damage Repair
-