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GPX4 antibody (C-Term)

Verified GPX4 Reactivity: Human WB, IHC, ELISA, IP Host: Goat Polyclonal unconjugated
Catalog No. ABIN185669
  • Target See all GPX4 Antibodies
    GPX4 (Glutathione Peroxidase 4 (GPX4))
    Binding Specificity
    • 8
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 46
    • 36
    • 31
    • 9
    • 3
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 47
    • 4
    Goat
    Clonality
    • 37
    • 14
    Polyclonal
    Conjugate
    • 25
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This GPX4 antibody is un-conjugated
    Application
    • 34
    • 17
    • 17
    • 8
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)
    Purpose
    GPX4 (Isoform a and c)
    Sequence
    EEPLVIEKDL PHY
    Specificity
    This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).
    Cross-Reactivity
    Cow, Human, Mouse, Pig, Rat
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-EEPLVIEKDLPHY, from the C Terminus of the protein sequence according to NP_002076.2, NP_001034937.1.
    Isotype
    IgG
    Top Product
    Discover our top product GPX4 Primary Antibody
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa. Recommended concentration: 0.3 μg/mL.

    Western Blot: Approx 19 kDa band observed in Human Kidney and Testis lysates and in lysates of cell line HEK293 (calculated MW of 22.1 kDa according to NP_002076.2). Recommended concentration: 0.1-0.3 μg/mL. This antibody has been successfully used in WB on

    Peptide ELISA: antibody detection limit dilution 1:16000.

    Comment

    Immunoprecipitation:This antibody has been successfully used in IP on Human: Schoenmakers E et al. (2010) PMID:21084748.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Papp, Lu, Bolderson, Boucher, Singh, Holmgren, Khanna: "SECIS-binding protein 2 promotes cell survival by protecting against oxidative stress." in: Antioxidants & redox signaling, Vol. 12, Issue 7, pp. 797-808, (2010) (PubMed).

    Schoenmakers, Agostini, Mitchell, Schoenmakers, Papp, Rajanayagam, Padidela, Ceron-Gutierrez, Doffinger, Prevosto, Luan, Montano, Lu, Castanet, Clemons, Groeneveld, Castets, Karbaschi, Aitken, Dixon et al.: "Mutations in the selenocysteine insertion sequence-binding protein 2 gene lead to a multisystem selenoprotein deficiency disorder in humans. ..." in: The Journal of clinical investigation, Vol. 120, Issue 12, pp. 4220-35, (2010) (PubMed).

  • Target
    GPX4 (Glutathione Peroxidase 4 (GPX4))
    Alternative Name
    GPX4 (GPX4 Products)
    Synonyms
    GPx-4 antibody, GSHPx-4 antibody, MCSP antibody, PHGPx antibody, snGPx antibody, snPHGPx antibody, Phgpx antibody, gpx-4 antibody, snGpx antibody, cb563 antibody, cb691 antibody, PHGPxa antibody, sb:cb563 antibody, zgc:92253 antibody, wu:fb82e03 antibody, 1700027O09Rik antibody, mtPHGPx antibody, ATGPX4 antibody, F11L15.5 antibody, glutathione peroxidase 4 antibody, GP-GPx4 antibody, glutathione peroxidase 4 antibody, glutathione peroxidase 4a antibody, GPX4 antibody, Gpx4 antibody, gpx4a antibody
    Background
    GPX4, glutathione peroxidase 4 (phospholipid hydroperoxidase) , HGNC:4556, PHGPx, snGPx, glutathione peroxidase 4, phospholipid hydroperoxidase, sperm nucleus glutathione peroxidase
    Gene ID
    2879
    NCBI Accession
    NP_002076, NP_001034937
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