ADAM10 antibody (AA 420-641)

Catalog No. ABIN1857886

Product Details anti-ADAM10 Antibody

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Binding Specificity: AA 420-641

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADAM10 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to A Disintegrin And Metalloprotease 10 (ADAM10)

Specificity: The antibody is a rabbit polyclonal antibody raised against ADAM10. It has been selected for its ability to recognize ADAM10 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant A Disintegrin And Metalloprotease 10 (ADAM10) corresdonding to Arg420~Val641 (Accession # O14672)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:350-1400 Immunohistochemistry: 5-20 μg/mL

1:35-140 Immunocytochemistry: 5-20 μg/mL

1:35-140 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for ADAM10

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Alternative Name: A Disintegrin And Metalloprotease 10 (ADAM10 Products)

Synonyms: ADAM10 antibody, Adam10 antibody, adam10 antibody, wu:fc03d12 antibody, ad10 antibody, cd156c antibody, kuz antibody, kuzbanian antibody, madm antibody, xadam10 antibody, ADAM-10 antibody, ADAM 10 antibody, AD10 antibody, CD156c antibody, HsT18717 antibody, MADM antibody, 1700031C13Rik antibody, LOC100219653 antibody, zgc:64203 antibody, ADAM metallopeptidase domain 10 antibody, ADAM metallopeptidase domain 10a antibody, ADAM metallopeptidase domain 10 S homeolog antibody, a disintegrin and metallopeptidase domain 10 antibody, ADAM metallopeptidase domain 10b antibody, ADAM10 antibody, Adam10 antibody, adam10a antibody, adam10.S antibody, adam10b antibody

Background: CD156-C, CD156c, MADM, Kuz, CDw156, Kuzbanian protein homolog, Mammalian disintegrin-metalloprotease

Pathways: Notch Signaling, EGFR Signaling Pathway