NPPA antibody (AA 25-152)

Catalog No. ABIN1858010

Product Details anti-NPPA Antibody

Target: NPPA (Natriuretic Peptide A (NPPA))

Binding Specificity: AA 25-152

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPPA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Atrial Natriuretic Peptide (ANP)

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQ GPLGSEF- NPVYSA VSNTDLMDFK NLLDHLEEKM PVEDEVMPPQ ALSEQTDEAG AALSSLSEVP PWTGEVNPSQ RDGGALGRGP WDPSDRSALL KSKLRALLAG PR

Specificity: The antibody is a rabbit polyclonal antibody raised against ANP. It has been selected for its ability to recognize ANP in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Atrial Natriuretic Peptide (ANP) corresdonding to Asn25~Arg152 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for NPPA

Target: NPPA (Natriuretic Peptide A (NPPA))

Alternative Name: Atrial Natriuretic Peptide (NPPA Products)

Synonyms: anp antibody, ANF antibody, ANP antibody, Pnd antibody, RATANF antibody, ATFB6 antibody, CDD-ANF antibody, PND antibody, NPPA antibody, Anf antibody, atrial natriuretic peptide antibody, natriuretic peptide A antibody, natriuretic peptide type A antibody, anp antibody, nppa antibody, NPPA antibody, Nppa antibody

Background: ANH, ANF, CDD, Atrial Natriuretic Factor, Atrial Natriuretic Hormone, Atriopeptin, Cardionatrine, Cardiodilatin