SMPD1 antibody (AA 319-579)

Catalog No. ABIN1858086

Product Details anti-SMPD1 Antibody

Target: SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))

Binding Specificity: AA 319-579

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMPD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Acid Sphingomyelinase (ASM)

Specificity: The antibody is a rabbit polyclonal antibody raised against ASM. It has been selected for its ability to recognize ASM in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Acid Sphingomyelinase (ASM) corresdonding to His319~Pro579 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SMPD1

Target: SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))

Alternative Name: Acid Sphingomyelinase (SMPD1 Products)

Synonyms: ASM antibody, ASMASE antibody, NPD antibody, A-SMase antibody, Zn-SMase antibody, aSMase antibody, SMPD1 antibody, sphingomyelin phosphodiesterase 1 antibody, sphingomyelin phosphodiesterase 1, acid lysosomal antibody, sphingomyelin phosphodiesterase antibody, SMPD1 antibody, Smpd1 antibody, LOC5578088 antibody

Background: SMPD1, NPD, SMase, aSMase, Sphingomyelin Phosphodiesterase 1,Acid Lysosomal, Simply Sphingomyelinase