MUC16 antibody (AA 21622-21762) (Biotin)

Catalog No. ABIN1858204

Product Details anti-MUC16 Antibody (Biotin)

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Binding Specificity: AA 21622-21762

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MUC16 antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- RVIALRSVK NGAETRVDLL CTYLQPLSGP GLPIKQVFHE LSQQTHGITR LGPYSLDKDS LYLNGYNEPG LDEPPTTPKP ATTFLPPLSE ATTAMGYHLK TLTLNFTISN LQYSPDMGKG SATFNSTEGV LQHLLRPLFQ KS

Specificity: It has been selected for its ability to recognize CA125 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant CA125 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against CA125 conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant CA125 (Arg21622~Ser21762) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for MUC16

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Alternative Name: Carbohydrate Antigen 125 (CA125) (CA125 Products)

Synonyms: CA125 antibody, 1110008I14Rik antibody, Ca125 antibody, Gm1480 antibody, RGD1308422 antibody, MUC16 antibody, mucin 16, cell surface associated antibody, mucin 16 antibody, mucin-16 antibody, MUC16 antibody, Muc16 antibody, LOC476718 antibody, LOC712735 antibody