Chemerin antibody (AA 25-152)

Catalog No. ABIN1858398

Product Details anti-Chemerin Antibody

Target: Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))

Binding Specificity: AA 25-152

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Chemerin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Chemerin (CHEM)

Specificity: The antibody is a rabbit polyclonal antibody raised against CHEM. It has been selected for its ability to recognize CHEM in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Chemerin (CHEM) corresdonding to Ser25~Gly152 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Chemerin

Target: Chemerin (RARRES2) (Retinoic Acid Receptor Responder (Tazarotene Induced) 2 (RARRES2))

Alternative Name: Chemerin (RARRES2 Products)

Synonyms: HP10433 antibody, TIG2 antibody, 0610007L05Rik antibody, AI303516 antibody, retinoic acid receptor responder 2 antibody, retinoic acid receptor responder (tazarotene induced) 2 antibody, RARRES2 antibody, Rarres2 antibody

Background: TIG2, RARRES2, Tazarotene-Induced Gene 2, Retinoic Acid Receptor Responder 2,Tazarotene Induced, RAR-responsive protein TIG2

Pathways: Brown Fat Cell Differentiation