CMA1 antibody (AA 9-247)

Catalog No. ABIN1858430

Product Details anti-CMA1 Antibody

Target: CMA1 (Chymase 1, Mast Cell (CMA1))

Binding Specificity: AA 9-247

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CMA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Chymase 1, Mast Cell (CMA1)

Specificity: The antibody is a rabbit polyclonal antibody raised against CMA1. It has been selected for its ability to recognize CMA1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Chymase 1, Mast Cell (CMA1) corresdonding to Leu9~Asn247 (Accession # P21844)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-CMA1 antibody (ABIN1858430)

Germundson, Smith, Vendsel, Kelsch, Combs, Nagamoto-Combs: "Oral sensitization to whey proteins induces age- and sex-dependent behavioral abnormality and neuroinflammatory responses in a mouse model of food allergy: a potential role of mast cells." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 120, (2019) (PubMed).

Target Details for CMA1

Target: CMA1 (Chymase 1, Mast Cell (CMA1))

Alternative Name: Chymase 1, Mast Cell (CMA1 Products)

Synonyms: CYH antibody, MCT1 antibody, chymase antibody, MMCP-5 antibody, Mcp-5 antibody, Mcp5 antibody, Mcpt5 antibody, MCP3P antibody, CMA1 antibody, Chymase antibody, chymase 1 antibody, chymase 1, mast cell antibody, chymase-like antibody, CMA1 antibody, Cma1 antibody, LOC100008644 antibody

Background: CYH, CYM, Alpha-chymase, Mast cell protease I

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Carbohydrate Homeostasis