COL8A1 antibody (AA 590-744)
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- Target See all COL8A1 Antibodies
- COL8A1 (Collagen, Type VIII, alpha 1 (COL8A1))
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Binding Specificity
- AA 590-744
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Reactivity
- Rabbit
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Host
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Guinea Pig
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Clonality
- Polyclonal
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Conjugate
- This COL8A1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Sequence
- MGHHHHHHSG SEF-L GIDGVKTPHA YAAKKGKNGG PAYEMPAFTA ELTAPFPPVG APIKFDRLLY NGRQNYNPQT GIFTCEVPGV YYFAYHVHCK GGNVWVALFK NNEPVMYTYD EYKKGFLDQA SGSAVLLLRP GDRVFLQMPS EQAAGLYAGQ YVHSSFSGYL LYPM
- Specificity
- It has been selected for its ability to recognize COL8a1 in immunohistochemical staining and Western blotting.
- Purification
- Affinity Chromatography
- Immunogen
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Recombinant COL8a1 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against COL8a1. - Isotype
- IgG
- Top Product
- Discover our top product COL8A1 Primary Antibody
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- Application Notes
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Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Comment
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Content: The quality control contains recombinant COL8a1 (Leu590~Met744) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2% SDS, 200 mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Expiry Date
- 12 months
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- Target
- COL8A1 (Collagen, Type VIII, alpha 1 (COL8A1))
- Alternative Name
- Collagen Type VIII Alpha 1 (COL8A1 Products)
- Synonyms
- COL8A1 antibody, Col8a-1 antibody, C3orf7 antibody, collagen type VIII alpha 1 chain antibody, collagen, type VIII, alpha 1 S homeolog antibody, collagen, type VIII, alpha 1 antibody, COL8A1 antibody, col8a1.S antibody, Col8a1 antibody
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