CRADD antibody (AA 1-199)

Catalog No. ABIN1858488

Product Details anti-CRADD Antibody

Target: CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))

Binding Specificity: AA 1-199

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRADD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Monoclonal Antibody to CASP2 And RIPK1 Domain Containing Adaptor With Death Domain Protein (CRADD)

Sequence: MGHHHHHHSG SEF-MEARDKQVLR SLRLELGAEV LVEGLVLQYL YQEGILTENH IQEINAQTTG LRKTMLLLDI LPSRGPKAFD TFLDSLQEFP WVREKLKKAR EEAMTDLPAG DRLTGIPSHI LNSSPSDRQI NQLAQRLGPE WEPMVLSLGL SQTDIYRCKA NHPHNVQSQV VEAFIRWRQR FGKQATFQSL HNGLRAVEVD PSLLLHMLE

Specificity: The antibody is a mouse monoclonal antibody raised against CRADD. It has been selected for its ability to recognize CRADD in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant CASP2 And RIPK1 Domain Containing Adaptor With Death Domain Protein (CRADD) corresdonding to Met1~Glu199 (Accession # P78560) with N-terminal His Tag

Clone: 23#

Isotype: IgG2b kappa

Application Details

Application Notes:

Western blotting: 0.5-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-30 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-30 μg/mL Immunohistochemistry in paraffin section: 5-30 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CRADD

Target: CRADD (CASP2 and RIPK1 Domain Containing Adaptor with Death Domain (CRADD))

Alternative Name: CASP2 And RIPK1 Domain Containing Adaptor With Death Domain Protein (CRADD Products)

Synonyms: cradd antibody, MGC89035 antibody, zgc:101717 antibody, CRADD antibody, DKFZp468A2219 antibody, LOC100226369 antibody, raidd antibody, MGC78788 antibody, MRT34 antibody, RAIDD antibody, CASP2 and RIPK1 domain containing adaptor with death domain antibody, CASP2 and RIPK1 domain containing adaptor with death, gene 1 antibody, CASP2 and RIPK1 domain containing adaptor with death, gene 2 S homeolog antibody, CRADD antibody, cradd.1 antibody, cradd antibody, cradd.2.S antibody, Cradd antibody

Background: RAIDD, RIP-Associated ICH1/CED3-Homologous Protein With Death Domain, Caspase and RIP adapter with death domain, Death domain-containing protein CRADD

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity