Ezrin antibody (AA 1-295)

Catalog No. ABIN1858571

Product Details anti-Ezrin Antibody

Target: Ezrin (EZR)

Binding Specificity: AA 1-295

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ezrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Ezrin (EZR)

Specificity: The antibody is a rabbit polyclonal antibody raised against EZR. It has been selected for its ability to recognize EZR in immunohistochemical staining and western blotting.

Cross-Reactivity: Pig

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Ezrin (EZR) corresdonding to Met1~Arg295 (Accession # P15311)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:500-2000 Immunohistochemistry: 5-20 μg/mL

1:50-200 Immunocytochemistry: 5-20 μg/mL

1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Ezrin

Target: Ezrin (EZR)

Alternative Name: Ezrin (EZR Products)

Synonyms: CVIL antibody, CVL antibody, VIL2 antibody, ezr antibody, vil2 antibody, wu:fc05c03 antibody, wu:fj31d06 antibody, cvil antibody, cvl antibody, Vil2 antibody, AW146364 antibody, R75297 antibody, p81 antibody, ezr.S antibody, ezrin antibody, ezrin b antibody, ezrin L homeolog antibody, EZR antibody, ezrb antibody, ezr antibody, Ezr antibody, ezr.L antibody

Background: CVL, Ezrin, VIL2, CVIL, CVL, Villin 2, p81, Cytovillin

Pathways: Maintenance of Protein Location