HPRT1 antibody (AA 3-218)

Catalog No. ABIN1859195

Product Details anti-HPRT1 Antibody

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Binding Specificity: AA 3-218

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HPRT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1)

Specificity: The antibody is a rabbit polyclonal antibody raised against HPRT1. It has been selected for its ability to recognize HPRT1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) corresdonding to Thr3~Ala218 (Accession # P00492) with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-5 μg/mL Immunohistochemistry: 5-20 μg/mL Immunocytochemistry: 5-20 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for HPRT1

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Alternative Name: Hypoxanthine Phosphoribosyltransferase 1 (HPRT1 Products)

Synonyms: HGPRT antibody, HPRT antibody, C81579 antibody, HPGRT antibody, Hprt1 antibody, Hgprtase antibody, Hprt antibody, id:ibd1344 antibody, id:ibd5108 antibody, wu:fc10g09 antibody, zgc:56221 antibody, zgc:86608 antibody, hgprt antibody, hprt antibody, prtfdc1 antibody, hprt1 antibody, hprt1l antibody, zgc:55561 antibody, zgc:86771 antibody, hypoxanthine phosphoribosyltransferase 1 antibody, hypoxanthine guanine phosphoribosyl transferase antibody, hypoxanthine phosphoribosyltransferase 1 L homeolog antibody, phosphoribosyl transferase domain containing 1 antibody, HPRT1 antibody, Hprt antibody, Hprt1 antibody, hprt1 antibody, hprt1.L antibody, prtfdc1 antibody

Background: HGPRT, HGPRTase, HPRT, Lesch-Nyhan Syndrome, Hypoxanthine-guanine phosphoribosyltransferase

Pathways: Ribonucleoside Biosynthetic Process