JAG1 antibody (AA 470-834)

Catalog No. ABIN1859516

Product Details anti-JAG1 Antibody

Target: JAG1 (Jagged 1 (JAG1))

Binding Specificity: AA 470-834

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JAG1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Jagged 1 (JAG1)

Specificity: The antibody is a rabbit polyclonal antibody raised against JAG1. It has been selected for its ability to recognize JAG1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Jagged 1 (JAG1) corresdonding to Gly470~Thr834 (Accession # P78504)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for JAG1

Target: JAG1 (Jagged 1 (JAG1))

Alternative Name: Jagged 1 (JAG1 Products)

Synonyms: AGS antibody, AHD antibody, AWS antibody, CD339 antibody, HJ1 antibody, JAGL1 antibody, ABE2 antibody, Gsfabe2 antibody, Htu antibody, Ozz antibody, Ser-1 antibody, JAG1 antibody, jag3 antibody, cb243 antibody, jag1 antibody, sb:cb243 antibody, serrateC antibody, jagged1 antibody, serrate antibody, serrate-1 antibody, x-serrate-1 antibody, jagged 1 antibody, jagged 1b antibody, jagged 1a antibody, jagged 1 L homeolog antibody, JAG1 antibody, Jag1 antibody, jag1 antibody, jag1b antibody, jag1a antibody, jag1.L antibody

Background: CD339, AGS, AHD, AWS, HJ1, JAGL1, Alagille Syndrome, Jagged 1 Protein

Pathways: Notch Signaling, Stem Cell Maintenance