MMP13 antibody (AA 287-449)
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- Target See all MMP13 Antibodies
- MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))
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Binding Specificity
- AA 287-449
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Reactivity
- Rabbit
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Host
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Guinea Pig
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Clonality
- Polyclonal
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Conjugate
- This MMP13 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Sequence
- MGHHHHHHSG S- SLSL DAITSLRGET MIFKDRFFWR LHPQQVDAEL FLTKSFWPEL PNRIDAAYEH PARDLIFIFR GKKFWAPNGY DILEGYPQKL SELGFPREVK KISAAVHFED TGKTLFFSGN QVWSYDDTNH TMDQDYPRLI EEEFPGIGGK VDAVYEKNGY IYFFNGPIQ
- Specificity
- It has been selected for its ability to recognize MMP13 in immunohistochemical staining and Western blotting.
- Purification
- Affinity Chromatography
- Immunogen
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Recombinant MMP13 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against MMP13. - Isotype
- IgG
- Top Product
- Discover our top product MMP13 Primary Antibody
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- Application Notes
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Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Comment
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Content: The quality control contains recombinant MMP13 (Ser287~Gln449) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % . - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Expiry Date
- 12 months
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- Target
- MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))
- Alternative Name
- Matrix Metalloproteinase 13 (MMP13 Products)
- Synonyms
- CLG3 antibody, MANDP1 antibody, Clg antibody, MMP-13 antibody, Mmp1 antibody, cb1034 antibody, mmp13 antibody, gene A antibody, xCol antibody, xcl3 antibody, MMP13 antibody, MGC108008 antibody, matrix metallopeptidase 13 antibody, matrix metallopeptidase 13a antibody, matrix metallopeptidase 13 (collagenase 3) like S homeolog antibody, matrix metallopeptidase 13 (collagenase 3) antibody, matrix metalloproteinase 13 antibody, matrix metallopeptidase 13 (collagenase 3) like L homeolog antibody, MMP13 antibody, Mmp13 antibody, mmp13a antibody, mmp13l.S antibody, mmp13 antibody, LOC100136348 antibody, mmp13l.L antibody
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