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PDGF-BB Homodimer (AA 82-190) antibody

Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal 4# unconjugated
Catalog No. ABIN1860158
  • Target
    PDGF-BB Homodimer
    Binding Specificity
    • 15
    • 8
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    AA 82-190
    Reactivity
    • 39
    • 25
    • 21
    • 15
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 42
    • 4
    • 1
    Mouse
    Clonality
    • 43
    • 4
    Monoclonal
    Conjugate
    • 20
    • 7
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Un-conjugated
    Application
    • 47
    • 17
    • 13
    • 13
    • 11
    • 6
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    Purpose
    Monoclonal Antibody to Platelet Derived Growth Factor BB (PDGF BB)
    Sequence
    MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- SLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT
    Specificity
    The antibody is a mouse monoclonal antibody raised against PDGF BB. It has been selected for its ability to recognize PDGF BB in immunohistochemical staining and western blotting.
    Cross-Reactivity
    Mouse, Rat
    Purification
    Protein A + Protein G affinity chromatography
    Immunogen
    Recombinant Platelet Derived Growth Factor BB (PDGF BB) corresdonding to Ser82~Thr190 with N-terminal His Tag
    Clone
    4#
    Isotype
    IgG2b kappa
  • Application Notes

    Western blotting: 0.5-2 μg/mL

    1:500-2000 Immunohistochemistry: 5-20 μg/mL

    1:50-200 Immunocytochemistry: 5-20 μg/mL

    1:50-200 Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freeze/thaw cycles
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Target
    PDGF-BB Homodimer
    Alternative Name
    Platelet Derived Growth Factor BB
    Synonyms
    PDGF-2 antibody, PDGF2 antibody, SIS antibody, SSV antibody, c-sis antibody, PDGF-B antibody, Sis antibody, platelet derived growth factor subunit B antibody, platelet derived growth factor, B polypeptide antibody, PDGFB antibody, Pdgfb antibody
    Background
    PDGF-BB
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