AKT3 antibody (AA 46-338)
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- Target See all AKT3 Antibodies
- AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))
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Binding Specificity
- AA 46-338
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This AKT3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Protein Kinase B Gamma (PKBg)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against PKBg. It has been selected for its ability to recognize PKBg in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Mouse, Pig, Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Protein Kinase B Gamma (PKBg) corresdonding to Asp46~Glu338 with N-terminal His Tag
- Isotype
- IgG
- Top Product
- Discover our top product AKT3 Primary Antibody
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- Application Notes
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Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))
- Alternative Name
- Protein Kinase B Gamma (AKT3 Products)
- Synonyms
- MPPH antibody, PKB-GAMMA antibody, PKBG antibody, PRKBG antibody, RAC-PK-gamma antibody, RAC-gamma antibody, STK-2 antibody, AI851531 antibody, D930002M15Rik antibody, Nmf350 antibody, Pkbg antibody, AKT serine/threonine kinase 3 antibody, thymoma viral proto-oncogene 3 antibody, v-akt murine thymoma viral oncogene homolog 3b antibody, AKT3 antibody, Akt3 antibody, akt3b antibody
- Background
- PKBG, PRKB-G, AKT3, RAC-PK-Gamma, RAC-Gamma, STK2, RAC-Gamma Serine/Threonine-Protein Kinase, V-Akt Murine Thymoma Viral Oncogene Homolog 3
- Pathways
- PI3K-Akt Signaling, RTK Signaling, TLR Signaling, Hepatitis C, VEGF Signaling
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