SMAD5 antibody (AA 210-464)

Catalog No. ABIN1860592

Product Details anti-SMAD5 Antibody

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Binding Specificity: AA 210-464

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Purpose: Polyclonal Antibody to SMAD family member 5 (SMAD5)

Specificity: The antibody is a rabbit polyclonal antibody raised against SMAD5. It has been selected for its ability to recognize SMAD5 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant SMAD family member 5 (SMAD5) corresdonding to Gly210~Val464 (Accession # Q99717)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:500-2000 Immunohistochemistry: 5-20 μg/mL

1:50-200 Immunocytochemistry: 5-20 μg/mL

1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SMAD5

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Alternative Name: SMAD family member 5 (SMAD5 Products)

Synonyms: DWFC antibody, JV5-1 antibody, MADH5 antibody, Madh5 antibody, SBN antibody, fb67b04 antibody, mad antibody, madh5 antibody, sb:cb400 antibody, smd5 antibody, wu:fb67b04 antibody, SMAD5 antibody, DKFZp468M0619 antibody, 1110051M15Rik antibody, AI451355 antibody, Dwf-C antibody, MusMLP antibody, SMAD family member 5 antibody, SMAD5 antibody, Smad5 antibody, smad5 antibody

Background: MADH5, Dwfc, JV5-1, Mothers Against Decapentaplegic Homolog 5