TAF1 antibody (AA 1379-1636)

Catalog No. ABIN1860674

Product Details anti-TAF1 Antibody

Target: TAF1 (TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor (TAF1))

Binding Specificity: AA 1379-1636

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TAF1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to TATA Box Binding Protein Associated Factor 1 (TAF1)

Specificity: The antibody is a rabbit polyclonal antibody raised against TAF1. It has been selected for its ability to recognize TAF1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant TATA Box Binding Protein Associated Factor 1 (TAF1) corresdonding to Thr1379~Pro1636 (Accession # P21675)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for TAF1

Target: TAF1 (TAF1 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor (TAF1))

Alternative Name: TATA Box Binding Protein Associated Factor 1 (TAF1 Products)

Synonyms: 250kDa antibody, AU015687 antibody, B430306D02Rik antibody, Ccg-1 antibody, Ccg1 antibody, KAT4 antibody, N-TAF1 antibody, TAFII250 antibody, Taf2a antibody, BA2R antibody, CCG1 antibody, CCGS antibody, DYT3 antibody, DYT3/TAF1 antibody, NSCL2 antibody, OF antibody, P250 antibody, TAF2A antibody, XDP antibody, TATA-box binding protein associated factor 1 antibody, Taf1 antibody, TAF1 antibody

Background: NSCL2, OF, BA2R, CCG1, CCGS, P250, TAF2A, KAT4, DYT3/TAF1, TAF1 RNA Polymerase II, Dystonia 3, TBP-associated factor 250 kDa, Cell cycle gene 1 protein