TGM1 antibody (AA 433-692)

Catalog No. ABIN1860740

Product Details anti-TGM1 Antibody

Target: TGM1 (Transglutaminase 1, Keratinocyte (TGM1))

Binding Specificity: AA 433-692

Reactivity: Rabbit

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This TGM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Sequence: MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQ GPLGSEF- MDIYFDEN MKPLEHLNRD SVWNFHVWND CWMKRPDLPS GFDGWQVVDA TPQETSSGIF CCGPCSVESV KNGLVYMKYD TPFIFAEVNS DKVYWQRQDD GSFKIVYVEE KAIGTLIVTK AVRSHMREDI THIYKHPEGS DAERKAVETA AAHGSKPNVY DSRDSAEDVA MQVEAQDAVM GQDLTVSVVL TNRSSSRRTV KLHLYLSVTF YTGVTGSIFK ESKKEVVLAA GSSDSVVMPV AYKEYRPHLV DQGAMLLNVS GH

Specificity: It has been selected for its ability to recognize TGM1 in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant TGM1 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against TGM1.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant TGM1 (Met433~His692) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2% SDS, 200 mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for TGM1

Target: TGM1 (Transglutaminase 1, Keratinocyte (TGM1))

Abstract: TGM1 Products

Synonyms: TGM1 antibody, ARCI1 antibody, ICR2 antibody, KTG antibody, LI antibody, LI1 antibody, TGASE antibody, TGK antibody, 2310004J08Rik antibody, Tgase1 antibody, transglutaminase 1 antibody, transglutaminase 1, K polypeptide antibody, Tgm1 antibody, TGM1 antibody