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TNF alpha antibody (AA 63-233)

TNF alpha Reactivity: Dog WB, ELISA, ICC, IHC (p), IHC (fro) Host: Mouse Monoclonal unconjugated
Catalog No. ABIN1860785
  • Target See all TNF alpha Antibodies
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Binding Specificity
    • 47
    • 32
    • 15
    • 15
    • 14
    • 13
    • 10
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 63-233
    Reactivity
    • 324
    • 182
    • 123
    • 67
    • 50
    • 42
    • 27
    • 26
    • 23
    • 16
    • 12
    • 10
    • 10
    • 9
    • 6
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Dog
    Host
    • 262
    • 193
    • 31
    • 25
    • 6
    • 2
    • 2
    Mouse
    Clonality
    • 278
    • 234
    Monoclonal
    Conjugate
    • 249
    • 86
    • 45
    • 20
    • 17
    • 12
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This TNF alpha antibody is un-conjugated
    Application
    • 348
    • 210
    • 129
    • 122
    • 74
    • 62
    • 58
    • 49
    • 48
    • 40
    • 39
    • 38
    • 37
    • 32
    • 20
    • 12
    • 10
    • 9
    • 9
    • 6
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Sequence
    MGHHHHHHSG SEF-LPNGLQLI SPLAQTVKSS SRTPSDKPVA HVVANPEAEG QLQWLSRRAN ALLANGVELT DNQLIVPSDG LYLIYSQVLF KGQGCPSTHV LLTHTISRFA VSYQTKVNLL SAIKSPCQRE TPEGTEAKPW YEPIYLGGVF QLEKGDRLSA EINLPNYLDF AESGQVYFGI IAL
    Specificity
    It has been selected for its ability to recognize TNFa in immunohistochemical staining and Western blotting.
    Purification
    Affinity Chromatography
    Immunogen
    TNFa (AA 63-233)
    Isotype
    IgG
    Top Product
    Discover our top product TNF alpha Primary Antibody
  • Application Notes
    Western blotting: 1:100-400
    Immunocytochemistry in formalin fixed cells: 1:100-500
    Immunohistochemistry in formalin fixed frozen section: 1:100-500
    Immunohistochemistry in paraffin section: 1:50-200
    Enzyme-linked Immunosorbent Assay: 1:100-200
    Optimal working dilutions must be determined by end user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freeze/thaw cycles
    Storage
    4 °C
    Storage Comment
    Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
    Expiry Date
    12 months
  • Target
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Alternative Name
    Tumor Necrosis Factor Alpha (TNFa) (TNF alpha Products)
    Synonyms
    DIF antibody, TNF-alpha antibody, TNFA antibody, TNFSF2 antibody, RATTNF antibody, Tnfa antibody, tnf antibody, TNF-a antibody, TNFalpha antibody, Tnfsf1a antibody, TNFa antibody, cTNF antibody, Tnf-alpha antibody, tnfa-like antibody, TNF-ALPHA antibody, dif antibody, tnfa antibody, xtnf antibody, tnfsf2 antibody, tnf-alpha antibody, Cachectin antibody, tumor necrosis factor antibody, tumor necrosis factor b (TNF superfamily, member 2) antibody, tumor necrosis factor alpha antibody, tumor necrosis factor a (TNF superfamily, member 2) antibody, TNF antibody, Tnf antibody, tnf antibody, tnfb antibody, tnf-alpha antibody, LOC103694380 antibody, tnfa antibody
    Pathways
    NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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