Apelin antibody (AA 23-77)
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- Target See all Apelin (APLN) Antibodies
- Apelin (APLN)
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Binding Specificity
- AA 23-77
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Reactivity
- Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Apelin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Apelin (APLN)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against APLN. It has been selected for its ability to recognize APLN in immunohistochemical staining and western blotting.
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Apelin (APLN) corresdonding to Val23~Phe77 with N-terminal His and GST Tag
- Isotype
- IgG
- Top Product
- Discover our top product APLN Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- Apelin (APLN)
- Alternative Name
- Apelin (APLN Products)
- Synonyms
- APLN antibody, Xapelin antibody, xnpep2 antibody, preproapelin antibody, 6030430G11Rik antibody, Apel antibody, APEL antibody, XNPEP2 antibody, apelin antibody, apelin S homeolog antibody, apln antibody, APLN antibody, apln.S antibody, Apln antibody
- Background
- XNPEP2, APEL, APJ endogenous ligand
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Hormone Activity, Feeding Behaviour
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