C4A antibody (AA 678-753)

Catalog No. ABIN1862265

Product Details anti-C4A Antibody

Target: C4A (C4a) (Complement Fragment C4a (C4a))

Binding Specificity: AA 678-753

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C4A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Complement Component 4a (C4a)

Specificity: The antibody is a rabbit polyclonal antibody raised against C4a. It has been selected for its ability to recognize C4a in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Complement Component 4a (C4a) corresdonding to Asn678~Arg753 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:120-480 Immunohistochemistry: 5-20 μg/mL

1:12-48 Immunocytochemistry: 5-20 μg/mL

1:12-48 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for C4A

Target: C4A (C4a) (Complement Fragment C4a (C4a))

Alternative Name: Complement Component 4a (C4a Products)

Synonyms: C4 antibody, C4A2 antibody, C4A3 antibody, C4A4 antibody, C4A6 antibody, C4AD antibody, C4S antibody, CO4 antibody, CPAMD2 antibody, RG antibody, Slp antibody, C4-1 antibody, C4b antibody, complement C4A (Rodgers blood group) antibody, complement component 4A (Rodgers blood group) antibody, C4A antibody, C4a antibody

Background: C4a anaphylatoxin

Pathways: Complement System