SEMA3E antibody (AA 32-516)

Catalog No. ABIN1863140

Product Details anti-SEMA3E Antibody

Target: SEMA3E (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E))

Binding Specificity: AA 32-516

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEMA3E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Semaphorin 3E (SEMA3E)

Specificity: The antibody is a rabbit polyclonal antibody raised against SEMA3E. It has been selected for its ability to recognize SEMA3E in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Semaphorin 3E (SEMA3E) corresdonding to Arg32~Phe516 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for SEMA3E

Target: SEMA3E (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E))

Alternative Name: Semaphorin 3E (SEMA3E Products)

Synonyms: M-SEMAH antibody, M-SemaK antibody, SEMAH antibody, coll-5 antibody, 6430702L12 antibody, AA408817 antibody, Semah antibody, mKIAA0331 antibody, semaphorin 3E antibody, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E antibody, SEMA3E antibody, Sema3e antibody

Background: SEMAH, M-SemaK, M-sema H, coll-5, Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3E