ALOX15B antibody (AA 253-490)

Catalog No. ABIN1866642

Product Details anti-ALOX15B Antibody

Target: ALOX15B (Arachidonate 15-Lipoxygenase B (ALOX15B))

Binding Specificity: AA 253-490

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX15B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to 15-Lipoxygenase-2 (15-LO-2)

Specificity: The antibody is a rabbit polyclonal antibody raised against 15-LO-2. It has been selected for its ability to recognize 15-LO-2 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant 15-Lipoxygenase-2 (15-LO-2) corresdonding to Arg253~Glu490 (Accession # O15296)

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for ALOX15B

Target: ALOX15B (Arachidonate 15-Lipoxygenase B (ALOX15B))

Alternative Name: 15-Lipoxygenase-2 (ALOX15B Products)

Synonyms: 15-LOX-2 antibody, ALOX15B antibody, arachidonate 15-lipoxygenase, type B antibody, arachidonate 15-lipoxygenase B antibody, ALOX15B antibody, Alox15b antibody, LOC100525835 antibody

Background: ALOX15B, LOX15B, 15-LOX-2, Arachidonate 15-lipoxygenase type II, Lipoxygenase 15, Type B, 15-lipoxygenase 2, Linoleate 13-lipoxygenase 15-LOb Arachidonate-15-Lipoxygenase, Type B