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APAF1 antibody (AA 31-350)

APAF1 Reactivity: Human WB, ELISA, IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1866706
  • Target See all APAF1 Antibodies
    APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))
    Binding Specificity
    • 16
    • 15
    • 9
    • 8
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 31-350
    Reactivity
    • 84
    • 51
    • 50
    • 15
    • 1
    Human
    Host
    • 71
    • 8
    • 7
    • 1
    Rabbit
    Clonality
    • 64
    • 23
    Polyclonal
    Conjugate
    • 43
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This APAF1 antibody is un-conjugated
    Application
    • 52
    • 27
    • 26
    • 26
    • 21
    • 15
    • 14
    • 13
    • 13
    • 11
    • 9
    • 3
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Purpose
    Polyclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1)
    Specificity
    The antibody is a rabbit polyclonal antibody raised against APAF1. It has been selected for its ability to recognize APAF1 in immunohistochemical staining and western blotting.
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    Recombinant Apoptotic Peptidase Activating Factor 1 (APAF1) corresdonding to Ser31~Phe350 (Accession # O14727)
    Isotype
    IgG
    Top Product
    Discover our top product APAF1 Primary Antibody
  • Application Notes

    Western blotting: 0.5-2 μg/mL

    Immunohistochemistry: 5-20 μg/mL

    Immunocytochemistry: 5-20 μg/mL

    Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    Preservative
    ProClin
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Target
    APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))
    Alternative Name
    Apoptotic Peptidase Activating Factor 1 (APAF1 Products)
    Synonyms
    APAF1 antibody, ARK antibody, Apaf-1 antibody, Apaf1 antibody, CG6829 antibody, D-Apaf-1 antibody, DARK antibody, Dapaf-1 antibody, Dapaf-1/HAC-1 antibody, Dark antibody, Dark/Apaf-I antibody, Dark/Dapaf-1/HAC1 antibody, Dark/Hac-1/dApaf-1 antibody, Dark/Hac-1/dApaf1 antibody, Dmel\\CG6829 antibody, Hac-1 antibody, Hac-1/Dark antibody, Hac1 antibody, T1 antibody, anon-53Fa antibody, apaf-1 antibody, apaf1 antibody, arc antibody, ark antibody, dAPAF-1 antibody, dApaf-1 antibody, dApaf-1/DARK/HAC-1 antibody, dApaf1 antibody, dArk antibody, dapaf antibody, dapaf-1 antibody, dapaf-1L antibody, dapaf-1S antibody, dark antibody, dark/dapaf-1/hac-1 antibody, dark/hac-1/dapaf-1 antibody, hac-1 antibody, hac1 antibody, l(2)SH0173 antibody, APAF-1 antibody, CED4 antibody, 6230400I06Rik antibody, Apaf1l antibody, fog antibody, mKIAA0413 antibody, zgc:136543 antibody, Death-associated APAF1-related killer antibody, apoptotic peptidase activating factor 1 L homeolog antibody, apoptotic peptidase activating factor 1 antibody, Dark antibody, apaf1.L antibody, apaf1 antibody, APAF1 antibody, Apaf1 antibody
    Background
    CED4, Apoptotic Protease Activating Factor
    Pathways
    p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity
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