Calpain 2 antibody (AA 45-514)

Catalog No. ABIN1867019

Product Details anti-Calpain 2 Antibody

Target: Calpain 2 (CAPN2)

Binding Specificity: AA 45-514

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calpain 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Calpain 2 (CAPN2)

Specificity: The antibody is a rabbit polyclonal antibody raised against CAPN2. It has been selected for its ability to recognize CAPN2 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Calpain 2 (CAPN2) corresdonding to Leu45~Asp514 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:300-1200 Immunohistochemistry: 5-20 μg/mL

1:30-120 Immunocytochemistry: 5-20 μg/mL

1:30-120 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Calpain 2

Target: Calpain 2 (CAPN2)

Alternative Name: Calpain 2 (CAPN2 Products)

Synonyms: CANP2 antibody, CANPL2 antibody, CANPml antibody, mCANP antibody, AI326419 antibody, Capa-2 antibody, Capa2 antibody, m-calpain antibody, m-calpin antibody, capn2 antibody, CAPN2 antibody, im:6908877 antibody, zgc:109906 antibody, calpain 2 antibody, calpain 2, gene2 antibody, calpain 2, (m/II) large subunit b antibody, CAPN2 antibody, Capn2 antibody, capn2.2 antibody, capn2b antibody

Background: CANPL2, CANPml, mCANP, Calpain-2 Catalytic Subunit, Calcium-activated neutral proteinase 2, Calpain M-type, Calpain large polypeptide L2, Millimolar-calpain, Calpain 2, Large Subunit