Calpain 3 antibody (AA 602-821)

Catalog No. ABIN1867020

Product Details anti-Calpain 3 Antibody

Target: Calpain 3 (CAPN3)

Binding Specificity: AA 602-821

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calpain 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Calpain 3 (CAPN3)

Specificity: The antibody is a rabbit polyclonal antibody raised against CAPN3. It has been selected for its ability to recognize CAPN3 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Calpain 3 (CAPN3) corresdonding to Ile602~Ala821

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Calpain 3

Target: Calpain 3 (CAPN3)

Alternative Name: Calpain 3 (CAPN3 Products)

Synonyms: capn3 antibody, MGC80925 antibody, zgc:92687 antibody, CAPN3 antibody, AI323605 antibody, Capa-3 antibody, Capa3 antibody, Lp82 antibody, p94 antibody, Lp84 antibody, Lp85 antibody, CANP3 antibody, CANPL3 antibody, LGMD2 antibody, LGMD2A antibody, nCL-1 antibody, SKM-CALP antibody, SKM-CALPOV antibody, nCL1 antibody, calpain 1, (mu/I) large subunit L homeolog antibody, calpain 3a, (p94) antibody, calpain 3 antibody, capn1.L antibody, capn3a antibody, CAPN3 antibody, Capn3 antibody

Background: CANPL3, p94, LGMD2, LGMD2A, nCL1, Calcium-activated neutral proteinase 3, Muscle-specific calcium-activated neutral protease 3, New calpain 1

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development