CENPF antibody (AA 436-637)

Catalog No. ABIN1867182

Product Details anti-CENPF Antibody

Target: CENPF (Centromere Protein F (CENPF))

Binding Specificity: AA 436-637

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPF antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Polyclonal Antibody to Centromere Protein F (CENPF)

Specificity: The antibody is a rabbit polyclonal antibody raised against CENPF. It has been selected for its ability to recognize CENPF in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Centromere Protein F (CENPF) corresdonding to Gln436~Lys637 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CENPF

Target: CENPF (Centromere Protein F (CENPF))

Alternative Name: Centromere Protein F (CENPF Products)

Synonyms: LOC100222985 antibody, 6530404A22Rik antibody, AI325968 antibody, CENF antibody, Lek1 antibody, PRO1779 antibody, mitosin antibody, im:7140452 antibody, im:7144238 antibody, im:7154719 antibody, im:7156761 antibody, si:ch211-152h14.2 antibody, hcp-1 antibody, CMF1 antibody, centromere protein F antibody, CENPF antibody, Cenpf antibody, cenpf antibody

Background: CENP-F, CENF, Hcp-1, Centromere protein F(350/400kD,Mitosin), AH antigen, Kinetochore protein CENPF, Mitosin

Pathways: Chromatin Binding, M Phase, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection