Esophageal Cancer Related Gene 4 (AA 32-148) antibody

Catalog No. ABIN1867688

Product Details

Target: Esophageal Cancer Related Gene 4

Binding Specificity: AA 32-148

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)

Purpose: Polyclonal Antibody to Esophageal Cancer Related Gene 4 (ECRG4)

Specificity: The antibody is a rabbit polyclonal antibody raised against ECRG4. It has been selected for its ability to recognize ECRG4 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Esophageal Cancer Related Gene 4 (ECRG4) corresdonding to Asn32~Tyr148 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:330-1300 Immunohistochemistry: 5-20 μg/mL

1:33-130 Immunocytochemistry: 5-20 μg/mL

1:33-130 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: Esophageal Cancer Related Gene 4

Synonyms: ECRG4 antibody, chromosome 2 open reading frame 40 antibody, C2orf40 antibody

Background: C2orf40, Augurin