EEA1 antibody (AA 1232-1348)

Catalog No. ABIN1867692

Product Details anti-EEA1 Antibody

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Binding Specificity: AA 1232-1348

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EEA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Early Endosome Antigen 1 (EEA1)

Specificity: The antibody is a rabbit polyclonal antibody raised against EEA1. It has been selected for its ability to recognize EEA1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Early Endosome Antigen 1 (EEA1) corresdonding to His1232~Lys1348 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:440-1750 Immunohistochemistry: 5-20 μg/mL

1:44-175 Immunocytochemistry: 5-20 μg/mL

1:44-175 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for EEA1

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Alternative Name: Early Endosome Antigen 1 (EEA1 Products)

Synonyms: MST105 antibody, MSTP105 antibody, ZFYVE2 antibody, im:7151048 antibody, si:ch211-276p10.2 antibody, A430109M19Rik antibody, B230358H09Rik antibody, early endosome antigen 1 antibody, early endosome antigen 1 S homeolog antibody, EEA1 antibody, eea1 antibody, Eea1 antibody, eea1.S antibody

Background: ZFYVE2, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2

Pathways: Toll-Like Receptors Cascades