Integrin beta 5 antibody (AA 137-378)

Catalog No. ABIN1868777

Product Details anti-Integrin beta 5 Antibody

Target: Integrin beta 5 (ITGB5)

Binding Specificity: AA 137-378

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Integrin beta 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Integrin Beta 5 (ITGb5)

Specificity: The antibody is a rabbit polyclonal antibody raised against ITGb5. It has been selected for its ability to recognize ITGb5 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Integrin Beta 5 (ITGb5) corresdonding to Pro137~Ile378 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

1:500-2000 Immunohistochemistry: 5-20 μg/mL

1:50-200 Immunocytochemistry: 5-20 μg/mL

1:50-200 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Integrin beta 5

Target: Integrin beta 5 (ITGB5)

Alternative Name: Integrin Beta 5 (ITGB5 Products)

Synonyms: ITGB5 antibody, AA475909 antibody, AI874634 antibody, ESTM23 antibody, [b]-5 antibody, [b]5 antibody, [b]5A antibody, [b]5B antibody, beta-5 antibody, beta5 antibody, RGD1563276 antibody, integrin, beta 5 antibody, integrin beta 5 antibody, integrin subunit beta 5 antibody, ITGB5 antibody, Itgb5 antibody

Background: ITG-B5

Pathways: Integrin Complex