MAN1A1 antibody (AA 322-653)

Catalog No. ABIN1869070

Product Details anti-MAN1A1 Antibody

Target: MAN1A1 (Mannosidase, Alpha, Class 1A, Member 1 (MAN1A1))

Binding Specificity: AA 322-653

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAN1A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Polyclonal Antibody to Mannosidase Alpha Class 1A Member 1 (MAN1A1)

Specificity: The antibody is a rabbit polyclonal antibody raised against MAN1A1. It has been selected for its ability to recognize MAN1A1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Pig, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Mannosidase Alpha Class 1A Member 1 (MAN1A1) corresdonding to Ile322~Glu653 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for MAN1A1

Target: MAN1A1 (Mannosidase, Alpha, Class 1A, Member 1 (MAN1A1))

Alternative Name: Mannosidase Alpha Class 1A Member 1 (MAN1A1 Products)

Synonyms: HUMM3 antibody, HUMM9 antibody, MAN9 antibody, Aa2-166 antibody, Man1a antibody, MAN1A antibody, si:dkey-164k19.1 antibody, si:dkeyp-6c7.1 antibody, Afu6g06790 antibody, AO090003000057 antibody, mannosidase alpha class 1A member 1 antibody, mannosidase, alpha, class 1A, member 1 antibody, mannosyl-oligosaccharide alpha-1,2-mannosidase antibody, Mannosyl-oligosaccharide alpha-1,2-mannosidase antibody, mannosyl-oligosaccharide alpha-1,2-mannosidase IA antibody, hypothetical protein antibody, MAN1A1 antibody, Man1a1 antibody, man1a1 antibody, AFUA_6G06790 antibody, TVAG_440710 antibody, LOC5578387 antibody, AOR_1_100154 antibody, CC1G_01001 antibody, PHATRDRAFT_52346 antibody, TSTA_047320 antibody, PGTG_11423 antibody

Background: HUMM3, HUMM9, MAN9, Mannosyl-Oligosaccharide 1,2-Alpha-Mannosidase IA, Mannosidase alpha class 1A member 1, Processing alpha-1,2-mannosidase IA