NRF2 antibody (AA 382-604)

Catalog No. ABIN1869445

Product Details anti-NRF2 Antibody

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Binding Specificity: AA 382-604

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NRF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2)

Specificity: The antibody is a rabbit polyclonal antibody raised against NFE2L2. It has been selected for its ability to recognize NFE2L2 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) corresdonding to Ala382~Asn604

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL

Immunohistochemistry: 5-20 μg/mL

Immunocytochemistry: 5-20 μg/mL

Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

References for anti-NRF2 antibody (ABIN1869445)

Baluchnejadmojarad, Zeinali, Roghani: "Scutellarin alleviates lipopolysaccharide-induced cognitive deficits in the rat: Insights into underlying mechanisms." in: International immunopharmacology, Vol. 54, pp. 311-319, (2018) (PubMed).

Target Details for NRF2

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Alternative Name: Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2 Products)

Synonyms: Nrf2 antibody, nfe2l2 antibody, wu:fc15g09 antibody, wu:fj67e03 antibody, NF-E2R2 antibody, NRF2 antibody, AI194320 antibody, nuclear factor, erythroid 2-like 2a antibody, nuclear factor, erythroid 2 like 2 S homeolog antibody, nuclear factor, erythroid 2 like 2 antibody, nuclear factor, erythroid derived 2, like 2 antibody, nuclear factor, erythroid 2-like 2 antibody, nfe2l2a antibody, nfe2l2.S antibody, NFE2L2 antibody, Nfe2l2 antibody

Background: NRF2, HEBP1, NF-E2-Related Factor 2, Nuclear factor, erythroid derived 2, like 2

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling