PCDH15 antibody

Catalog No. ABIN1869693

Product Details anti-PCDH15 Antibody

Target: PCDH15 (Protocadherin-15 (PCDH15))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCDH15 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Purpose: Polyclonal Antibody to Protocadherin 15 (PCDH15)

Specificity: The antibody is a rabbit polyclonal antibody raised against PCDH15. It has been selected for its ability to recognize PCDH15 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Protocadherin 15 (PCDH15) corresdonding to N-terminal His Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for PCDH15

Target: PCDH15 (Protocadherin-15 (PCDH15))

Alternative Name: Protocadherin 15 (PCDH15 Products)

Synonyms: CDHR15 antibody, DFNB23 antibody, USH1F antibody, BB078305 antibody, ENSMUSG00000046980 antibody, Gm9815 antibody, Ush1f antibody, av antibody, nmf19 antibody, protocadherin-15 antibody, protocadherin related 15 antibody, protocadherin-15 antibody, protocadherin 15 antibody, PCDH15 antibody, CpipJ_CPIJ005081 antibody, Pcdh15 antibody

Background: USH1F, DFNB23, CDHR15, Deafness,Autosomal Recessive 23, Cadherin-Related Family Member 15

Pathways: Sensory Perception of Sound